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AAT Bioquest

FAQ

Posted on January 16, 2024
What are the types of cell labeling?
FISHFlow Cytometry ReagentsMicroscopySpectral Flow Cytometryfluorescence microscopein situ hybridization


Posted on January 16, 2024
How do I label an animal cell?
Antibodies and ProteomicsMicroscopyfluorescence microscopeimmunofluorescence


Posted on January 16, 2024
What are the two most common types of microscopes?
Cellular Structures and OrganellesMicroscopyfluorescence microscope


Posted on December 7, 2023
What is super-resolution microscopy?
FluorescenceFluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the advantages of super resolution microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the limitations of super resolution microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the advantages of electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the limitations of electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the common super resolution microscopy techniques?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
How does super resolution microscopy work?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the applications of electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the applications of super resolution microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
Can super resolution imaging be used with live cells and tissues?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the factors to consider when choosing a super-resolution technique for my experiment?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
How do super resolution techniques overcome the challenges of diffraction limit?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the differences between super resolution and electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the common types of electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
How do the different types of electron microscopy work?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the differences between TEM and SEM (transmission vs scanning electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the differences between optical microscopy and electron microscopy?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on December 7, 2023
What are the differences between light microscope and electron microscope?
Fluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on April 13, 2023
What types of samples can be used with an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How should an antifade reagent be chosen for a specific experiment or analysis?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
Can antifade reagents be used in other types of imaging besides fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How long does the fluorescence signal last when using an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How do I reduce sample exposure in order to minimize photobleaching?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common anti-fading techniques?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common components of an antifade mounting medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What causes fading in fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on March 22, 2023
What are the applications of Ziehl-Neelsen staining?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the reagents used in the Ziehl-Neelsen stain?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the principle of the Kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the procedures of the kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the limitations of the Kinyoun-cold method?
FluorescenceGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What's the role of iodine in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the role of alcohol in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the fundamental differences between Ziehl-Neelsen technique and the Kinyoun technique?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the types of chemical reagents used in the staining process of the tissues?
Cellular Structures and OrganellesFluorescenceImmunohistochemistry (IHC)Organelle Stainingfluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What is the composition of the cell envelope?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
How do I distinguish gram negative and gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-negative cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-positive bacteria cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Is it easier to kill gram-positive bacteria than gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Which is more harmful, gram-positive bacteria or gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
Where are gram-positive bacteria found?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What is the significance of gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on July 22, 2020
What is fluorescence in situ hybridization (FISH)?
DNADNA and RNAFISHFluorescenceNucleic AcidsRNAfluorescence microscopein situ hybridization


Posted on June 1, 2020
What is the difference between bacteria and viruses?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What are acid-fast bacteria?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What are the common methods for bacteria counting?
Flow Cytometry ReagentsFluorescenceGram Stainingbacteriaflow cytometryfluorescence imagingfluorescence microscope


Posted on June 1, 2020
Are acid-fast bacteria gram positive or gram negative?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on May 9, 2020
What are natural killer cells?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)fluorescence microscopeimmunofluorescence


Posted on May 9, 2020
What is a buffy coat?
DNA extractionIHCImmunohistochemistry (IHC)centrifugefluorescence microscopeimmunofluorescence


Posted on April 24, 2020
Can antibodies for flow cytometry be used for fluorescence microscopy?
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope


Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization


Posted on April 17, 2020
What is the best fixation for fluorescence microscopy of GFP tagged proteins?
FixationPBSfixed cellsfluorescence microscope


Posted on April 17, 2020
How can I attach suspension cells to glass slides for fluorescence microscopy investigation?
FixationPBSfixed cellsfluorescence microscope Chemical Reagents


Posted on April 17, 2020
What is the difference between PLL and PDL for surface coating?
Cell CultureFixationadherent cellsfixed cells Chemical Reagentsfluorescence microscope


Posted on April 17, 2020
Which fluorescent substrate should I use for B-galactosidase system for microscopy?
β-galactosidaseEnzymesbeta-galactosidasefluorescence microscopeELISAEnzyme-Linked Immunosorbent Assay (ELISA)lac OperonlacZ


Posted on June 24, 2019
What is the working mechanism of MitoROS 580?
superoxideROS Probesmitochondria ROSflow cytometerfluorescence microscope


Posted on June 13, 2019
What is the mechanism of your Cell Meter™ Autophagy Assay Kits (cat#23000, 23001 and 23002)
Autophagylysosomefluorescence imagingfluorescence microscope


Posted on June 13, 2019
Does Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit support multiplex assays for detection of mitochondrial superoxide?
cell-permeable probesflow cytometryfluorescence microscopelive cell trackingmitochondriasuperoxide


Posted on June 13, 2019
Do your autophagy assay kits be used with a multi-function microplate reader to detect fluorescence intensity and reflect autophagy levels?
Autophagyfluorescence imagingfluorescence microscopeflow cytometer


Posted on June 13, 2019
Do you provide any dyes similar to CFSE that can track cells or monitor cell proliferation?
Cell Cytotoxicity iFluor dyesCytoTellcell proliferationcell-permeable probesflow cytometerfluorescence microplate readerfluorescence microscope


Posted on April 2, 2019
Can I treat my cells before adding the master reaction mix for ROS kit 22900
ROSfluorescence imagingfluorescent microscopefluorescence microscope


Posted on April 2, 2019
Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?
flow cytometerflow cytometryfluorescence imagingfluorescence microplate readerfluorescence microscopefluorescent plate reader


Posted on April 2, 2019
Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
Actin iFluor dyesCell AssayFixationF-actinFixativesfluorescence imagingfluorescence microscopephalloidin


Posted on January 3, 2019
Do you have any fixable mitochondria staining assay kits?
fixablemultiplexmitochondriafluorescence imagingfluorescence microscope


Posted on September 27, 2018
How do I stain T cells with CytoTell™ Blue?
Autophagylysosomefluorescence imagingfluorescence microscopecell proliferation


Posted on August 30, 2018
How do you stain paraffin-embedded tissue with a phalloidin"conjugate?
Phalloidinfluorescence imagingfluorescence microscope